GP130 siRNA明顯抑制 MDA-MB-231，SUM159和

GP130 siRNA明顯抑制 MDA-MB-231，SUM159和 MDA-MB-468的細胞活性，使GP130表達降低，及p-STAT3的下降。當使用Bazedoxifene 20μm時，P- STAT3、P- Akt和P- ERK均被抑制。但是STAT3在各細胞系中的表達量卻很大，且不受藥物治療的影響， 表示Bazedoxifene僅通過磷酸化抑制而不是抑制Stat3活化。IL-6能够升高P- STAT3，但當加入Bazedoxifene時，則會被抑制。Bazedoxifene對EGF最初刺激的P- STAT3、P- STAT5和P -Akt沒有抑制作用。結果顯示， Bazedoxifene可能通過靶向抑制IL-6/GP130發揮作用。

結果 (英文) 1:

GP130 siRNA significantly inhibited MDA-MB-231, SUM159 cell activity and MDA-MB-468, so that expression of GP130 reduced, and decrease of p-STAT3.

When using Bazedoxifene 20μm, P- STAT3, P- Akt and P- ERK were inhibited. However, STAT3 expression in the cell line is great, and is not affected by drug treatment, only by showing inhibition of phosphorylation of Bazedoxifene rather than inhibit activation of Stat3.

IL-6 can be raised P- STAT3, but when added Bazedoxifene, will be suppressed.

Bazedoxifene initial stimulation of EGF P- STAT3, P- STAT5 and P -Akt had no inhibitory effect. The results show, Bazedoxifene may IL-6 / GP130 by targeted inhibition play a role.

結果 (英文) 2:

GP130 siRNA significantly inhibits the cellular activity of MDA-MB-231, SUM159 and MDA-MB-468, resulting in reduced EXPRESSION of GP130 and a decrease in p-STAT3.

P-STAT3, P-Akt, and P-ERK are all suppressed when bazedoxifene is used. But STAT3 expressed large amounts in each cell line and was not affected by drug therapy, suggesting that Bazedoxifene inhibited, rather than inhibited, Stat3 activation by phosphorylation.

IL-6 can raise P-STAT3, but when Bazedoxifene is added, it is suppressed.

Bazedoxifene has no inhibition stoush on The P-STAT3, P-STAT5 and P-Akt that were initially stimulated by EGF. The results showed that Bazedoxifene may have acted through targeted suppression of IL-6/GP130.

結果 (英文) 3:

Gp130 siRNA significantly inhibited the cell activities of MDA-MB-231, sum159 and MDA-MB-468, and reduced the expression of gp130 and p-STAT3.
When bazedoxifene was used at 20 μ m, p-STAT3, p-Akt and p-ERK were all inhibited. However, the expression of STAT3 in each cell line was very large, and it was not affected by the drug treatment, indicating that bazedoxifene only inhibited STAT3 activation through phosphorylation inhibition.
IL-6 could increase p-STAT3, but it was inhibited when bazedoxifene was added.
Bazedoxifene did not inhibit the initial stimulation of p-STAT3, p-stat5 and p-Akt by EGF. The results showed that Bazedoxifene may play a role in the inhibition of IL-6/GP130.