We used 78 sequences from the fast-evolving nuclear internal tran-scribed spacers and are able to recognize 18 reciprocally monophyletic species. To address whether or not
porcini form a monophyletic group, we compiled a broadly sampled dataset of 41 taxa, including other
members of the Boletineae, and used separate and combined phylogenetic analysis of sequences from
the nuclear large subunit ribosomal DNA, the largest subunit of RNA polymerase II, and the mitochondrial
ATPase subunit six gene.