Similarly, for fraction II (Fig. 3B

Similarly, for fraction II (Fig. 3B), three consecutive1,4A-ions (1,4A2+Na at m/z 417,1,4A3+Na at m/z 563 and1,4A4+Na at m/z 709) demonstrated that all the glycosidic linkages are 3-linked.
The glycosidic fragment ions (B2+Na at m/z 473, C2+Na at m/z 491, B3+Na at m/z 619 and C3+Na at m/z 637) reflected the existence of two linked nonsulphated fucose residues, but the positions of the two sulphate groups are still ambiguous. For fraction III (Fig. 3C),1,4A-ions(1,4A2+Na at m/z 417,1,4A3at m/z 563,1,4A4+Na at m/z 709, and1,4A7+2Na at m/z 664)were found which suggested that fucose residues in fraction III are 3-linked. The glycosidic fragment ions(B6+2Na at m/z 628, B5+Na at m/z 911, and C5+Na at m/z 929)confirmed that a nonsulphated fucose residue exists at one terminal and that a disulsphated fucose residue is linked to it. The glycosidic fragment ions (B2+Na/C2+Na at m/z 473/491, B3+Na/C3+Na at m/z 619/637, and B4+Na/C4+Na at m/z 765/783) indicated the other two sulphate groups might exist at two fucose residues on the other terminal. The ions of fraction IV produced (Fig. 3D) were identical to those of fraction III, except for a glycosidic fragment ion (B7+ 2Na at m/z
692). This indicates that fraction IV consists of fraction III and a terminal nonsulphated fucose residue. ESI–CID–MS/MS provided critical information for the structural determination of the oligosaccharides, such as the nature of the glycosidic linkage, the partial sequence and the position of the sulphated residue. However, the NMR experiment was still necessary
in order to uncover the definitive structure

結果 (中文) 1:

Similarly, for fraction II (Fig. 3B), three consecutive1,4A-ions (1,4A2+Na at m/z 417,1,4A3+Na at m/z 563 and1,4A4+Na at m/z 709) demonstrated that all the glycosidic linkages are 3-linked.The glycosidic fragment ions (B2+Na at m/z 473, C2+Na at m/z 491, B3+Na at m/z 619 and C3+Na at m/z 637) reflected the existence of two linked nonsulphated fucose residues, but the positions of the two sulphate groups are still ambiguous. For fraction III (Fig. 3C),1,4A-ions(1,4A2+Na at m/z 417,1,4A3at m/z 563,1,4A4+Na at m/z 709, and1,4A7+2Na at m/z 664)were found which suggested that fucose residues in fraction III are 3-linked. The glycosidic fragment ions(B6+2Na at m/z 628, B5+Na at m/z 911, and C5+Na at m/z 929)confirmed that a nonsulphated fucose residue exists at one terminal and that a disulsphated fucose residue is linked to it. The glycosidic fragment ions (B2+Na/C2+Na at m/z 473/491, B3+Na/C3+Na at m/z 619/637, and B4+Na/C4+Na at m/z 765/783) indicated the other two sulphate groups might exist at two fucose residues on the other terminal. The ions of fraction IV produced (Fig. 3D) were identical to those of fraction III, except for a glycosidic fragment ion (B7+ 2Na at m/z692). This indicates that fraction IV consists of fraction III and a terminal nonsulphated fucose residue. ESI–CID–MS/MS provided critical information for the structural determination of the oligosaccharides, such as the nature of the glycosidic linkage, the partial sequence and the position of the sulphated residue. However, the NMR experiment was still necessary为了揭露 definitive 结构

結果 (中文) 3:

同样，对组分II（图3b），三consecutive1,4a-ions（1,4a2 Na m/z 417,1,4a3 Na在m/z 563 and1,4a4 Na m/z 709）表明所有的糖苷键是3。
糖苷碎片离子（B2 Na在m/z 473，m/z 491 C2 Na B3，Na在m/z 619和m/z 637 C3 NA）反映两存在联系nonsulphated岩藻糖残基，但两硫酸基团位置尚不明确。对于组分III（图3C），1,4a-ions（1,4a2 Na m/z 417,1,4a3at m/z 563,1,4a4 Na在m/z 709，and1,4a7 2Na m/z 664）发现提示第三岩藻糖基杆。糖苷（B6 2Na碎片离子m/z 628，m/z 911 B5 Na，和C5娜在m/z 929）CONfi出现在一个终端和一个disulsphated岩藻糖残基连接到它存在一个nonsulphated岩藻糖残基。糖苷的碎片离子（Na Na / B2 C2 m/z 473 / 491，B3 C3 NA NA / m / z为619 / 637，Na Na / C4和B4 m/z 765 / 783）表示，其他两组可能在两硫酸岩藻糖残基的另端存在。离子组分IV（图3d）产生了这些分数III相同，除了一个糖苷碎片离子（m/z
B7 2Na 692）。这表明，部分IV由分数III和终端nonsulphated岩藻糖残基。–CID–ESI MS / MS的寡糖结构分析提供了重要的信息，如糖苷键的性质，部分序列和硫酸渣的位置。然而，还是核磁共振实验是为了必要的
揭开defi认知结构