Samples were taken at 0, 0.5, 1.0,

Samples were taken at 0, 0.5, 1.0, 3.0 and 5.0 h and the reaction mixture was rapidly heated in a
boiling water bath for 30 min to inactivate the enzyme. The inactivated samples were submitted to
an extraction procedure according to Shore (1971) and histamine was quantified by a spectrofluorimetric method based on the reaction of histamine with o-phthalaldehyde (Shore, 1971).

結果 (中文) 1:

样品分别在 0、 0.5、 1.0、 3.0 和 5.0 h 和反应混合物被迅速加热中沸腾的水浴 30 分钟，使酶失活。灭活的样本提交给基于邻苯二甲醛 (岸，1971年) 与组胺反应的荧光分析法量化岸 (1971 年) 和组胺萃取过程。

結果 (中文) 3:

样品分别取为0，0.5，1，3和5小时，并在一个沸水浴30 min灭酶。灭活样品提交根据岸上的提取程序（1971）和组胺是由一个基于组胺与邻苯二甲醛反应光度法定量（岸，1971）。