To confirm whether the different pu

To confirm whether the different purple pigmentations
observed in the materials were attributable to the differential
accumulation of anthocyanins, the total anthocyanin content
was measured in the leaf tissue of two sets of seedlings. At
first, the purple parent p1029, the green parent g1025, and
the reddish-green F1 individuals were measured at the five￾leaf stage. When the BC3F2 was constructed, the total antho￾cyanin contents of individuals with differently colored leaves
in this population were measured. An ANOVA revealed
that in both samples, the differences between the anthocya￾nin contents in the purple, reddish-green and green leaves
were extremely significant (Online Resource Tables S2, S3).
Anthocyanin levels were almost three-fold higher in the leaf
tissues of purple plants than in those of reddish-green plants
(p1029: 0.633 ± 0.006 mg/g vs F1: 0.212 ± 0.016 mg/g;
PL: 0.552 ± 0.012 mg/g VS RGL: 0.189 ± 0.013 mg/g;
Fig. 2). Moreover, the anthocyanin content in the green
leaves was less than 50 % that of reddish-green plants
(g1025: 0.104 ± 0.006 mg/g vs F1: 0.212 ± 0.016 mg/g;
GL: 0.074 ± 0.007 mg/g vs RGL: 0.189 ± 0.013 mg/g). Our
results indicated that different shades of purple were corre￾lated with the anthocyanin content in the leaves.

結果 (中文) 1:

要确认是否不同的紫色沉着在观察到的材料被归因于差速器花色苷，总花色苷含量的积累测定两组幼苗叶片组织。在第一，紫色父 p1029，绿色父 g1025，和带红色绿色 F1 个人测定在五叶期。当构建了 BC3F2 时，与个人的总 antho 青内容不同色的叶子这一人群进行测定。方差分析显示在这两个样本中，anthocya 之间的差异万年内容中的紫色、 绿色的红的绿的叶子是极显著 (在线资源表 S2、 S3)。花色素苷含量几乎三倍高于叶紫色的植物比在那些红绿植物组织(p1029: 0.633 ± 0.006 毫克/克 vs F1: 0.212 ± 0.016 mg/g;PL: 0.552 ± 0.012 毫克/克 VS RGL: 0.189 ± 0.013 毫克/克;图 2）。此外，在绿色的花青苷含量叶子是低于 50%，红的绿的植物(g1025: 0.104 ± 0.006 毫克/克 vs F1: 0.212 ± 0.016 mg/g;GL: 0.074 ± 0.007 毫克/克 vs RGL: 0.189 ± 0.013 毫克/克)。我们结果表明，不同深浅的紫色相应与叶片中的花青素含量。

結果 (中文) 3:

确认不同的紫色色素沉着在材料中观察到的差异花色苷积累，总花色苷含量测定两组幼苗叶片组织。在首先，家长p1029紫色，绿色的母g1025，和在五￾叶期测定了红绿色的F1个体。当合构造，总花色苷含量显著￾用不同颜色的叶子的人在这一人群中进行测量。方差分析结果显示在这两种样品，在紫色的anthocya￾宁含量之间的差异，红色绿色和绿色的叶子有极显著意义（在线资源表S2，S3）。花青素含量几乎高出三倍的叶子紫色植物比红绿色植物的组织（p1029：0.633±0.006毫克/克VS F1：0.212±0.016 mg/g；PL：0.552±0.012毫克/克VS Rg1：0.189±0.013 mg/g；图2）。此外，绿色花青素含量叶片小于红绿色植物的50%（g1025：0.104±0.006毫克/克VS F1：0.212±0.016 mg/g；GL：0.074±0.007毫克/克VS Rg1：0.189±0.013毫克/克）。我们的结果表明，不同色调的紫色与￾含量与叶片中花青素的含量。