The gyrA and parC gene fragments of six E. coli isolates (F5, F8, F9, F13, F14 and F18) were amplified using previously described primers. 10,11 PCR amplimers were purified and processed for automated sequencing. Alignments of DNA sequences to reference gyrA and parC sequences, obtained from GenBank, and protein translations were performed using DNAMAN version 5.0 (Lynnon BioSoft, Canada)