From the trial table, all 6 formulas to make the backing layer layer found that the 1-5 formula has no physical characteristics as required, namely, the bubble is not flexible, peel off hard, so the formula 6 is the best and appropriate formula to put it into the most backing layer, since it meets the desired physical characteristics, is smooth. The thin and transparent sheet bubbles can be easily peel out from the petri dish. It has good elasticity, not easily lacking and no isolation layer of the sheet.
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