Recent studies have demonstrated th

Recent studies have demonstrated that long non-coding RNAs (lncRNAs) are regarded as useful tools for cancer detection, particularly for the early stage; however, little is known about their diagnostic impact on gastric cancer (GC). We hypothesized that GC-related lncRNAs might release into the circulation during tumor initiation and could be utilized to detect and monitor GC. 8 lncRNAs which previously found to be differently expressed in GC were selected as candidate targets for subsequent circulating lncRNA assay. After validating in 20 pairs of tissues and plasma in training set, H19 was selected for further analysis in another 70 patients and 70 controls. Plasma level of H19 was significantly higher in GC patients compared with normal controls (p < 0.0001). By receiver operating characteristic curve (ROC) analysis, the area under the ROC curve (AUC) was 0.838; p < 0.001; sensitivity, 82.9%; specificity, 72.9%). Furthermore, H19 expression enabled the differentiation of early stage GC from controls with AUC of 0.877; sensitivity, 85.5%; specificity, 80.1%. Besides, plasma levels of H19 were significantly lower in postoperative samples than preoperative samples (p = 0.001). In conclusion, plasma H19 could serve as a potential biomarker for diagnosis of GC, in particular for early tumor screening.

結果 (中文) 1:

最近的研究表明，长链非编码 Rna (lncRNAs) 被认为是有用的工具，为癌症检测，特别是早期的阶段;然而，鲜为人知的胃癌 (GC) 的诊断的影响。我们假设，GC 相关 lncRNAs 可能释放进入血液循环在肿瘤的发生和用于检测和监测 GC。以前发现在 GC 中以不同的方式表达的 8 lncRNAs 被选为后续循环 lncRNA 测定的候选目标。在验证之后 20 成对的组织及血浆中训练集，H19 被选为进一步分析在另一个 70 例患者和 70 的控件。H19 血浆水平显著高于 GC 患者与正常对照组 (p < 0.0001)。由接受者操作特征曲线 (ROC) 分析，(AUC) ROC 曲线下的面积是 0.838;p < 0.001;灵敏度，82.9%;特异性 72.9%）。此外，H19 表达启用早期胃癌从控件与 0.877; AUC 的分化灵敏度，85.5%;特异性，80.1%。此外，H19 血浆水平均在术后的样品明显低于术前样品 (p = 0.001)。总之，血浆 H19 可以作为潜在的生物标志物的 GC，诊断特别是早期肿瘤筛查。

結果 (中文) 3:

最近的研究表明，长非编码RNAs（lncRNAs）作为检测癌症的有用的工具，尤其是初级阶段；然而，很少是关于他们对胃癌诊断的影响（GC）。我们假设相关lncRNAs可能释放到循环中肿瘤的发生，可以用来检测和监测GC。8条此前发现有不同的表达在GC作为后续循环的lncRNA检测候选目标。经验证20对训练集的组织和血浆，H19被选定为进一步分析在70个患者和70名对照。H19血浆水平明显高于胃癌患者与正常对照组相比（P＜0.0001）。应用ROC曲线分析，ROC（ROC）的曲线下面积（AUC）为0.838；P＜0.001；敏感性，特异性72.9%），82.9%。此外，H19表达使分化的AUC为0.877控制早期的气相色谱法；灵敏度，特异性，80.1%，85.5%。此外，血浆H19明显低于较术前术后标本样品（P = 0.001）。总之，血浆H19可作为诊断胃癌的潜在生物标志物，特别是肿瘤早期筛查。