This study was to find an effective

This study was to find an effective process to extract bioactive peptides from mushroom foot and deter- mine their effects on activation of alcohol metabolic enzymes in vitro. The optimum extraction assisted by ultra-high-pressure processing of mushroom foot peptides was obtained with a pressure of 400 MPa and a processing time of 10 min. After ultrafiltration, peptides with molecular weight of 0–3 kDa had the highest activity to activate alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) by 70.79%and 71.35%, respectively. Following dextran gel chromatography, two peaks (p-I and p-II) appeared and the activation activities on ADH and ALDH of p-I were 72.00% and 73.43%, both higher than p-II. Nine pep- tides were found in p-I as determined by LC–MS/MS, and two of them (IPLH and IPIVLL) were synthe- sized. IPLH activated ADH and ALDH by 42.7% and 29.2% respectively, which were higher than IPIVLL.

結果 (繁體中文) 1:

這項研究是找到一個有效的過程，從蘑菇中提取足生物活性肽和阻止 - 礦體外酒精代謝酶的活化及其影響。用400MPa的壓力和10分鐘的處理時間得到的由腳菇肽的超高壓力加工輔助最佳提取。超濾後，用0-3 kDa的分子量的肽由70.79％要激活的醇脫氫酶（ADH）和醛脫氫酶（ALDH）活性最高
和71.35％之間。以下葡聚醣凝膠色譜法，兩個峰（PI和P-II）出現並在ADH和PI ALDH激活活動是72.00％和73.43％，比對 - II都更高。九pep-潮汐在pI值被發現通過LC-MS / MS確定，並且其中的兩個（IPLH和IPIVLL）為synthe-尺寸。IPLH分別激活ADH和ALDH由42.7％和29.2％，這比IPIVLL較高。

結果 (繁體中文) 2:

本研究旨在找到從蘑菇足中提取生物活性肽的有效工藝，並阻止它們對酒精代謝酶在體外啟動的影響。通過蘑菇足肽的超高壓加工獲得最佳萃取，壓力為400MPa，處理時間為10分鐘。超濾後，分子量為0~3 kDa的肽活性最高，啟動酒精脫氫酶（ADH）和醛脫氫酶（ALDH）的活性為70.79%
和71.35%。在dextran凝膠色譜調查後，出現兩個峰（p-I和p-II），p-IADH和ALDH的活化活動為72.00%和73.43%，均高於p-II。在LC_MS/MS測定的p-I中發現9個肽，其中兩個（IPLH和IPIVLL）是合成大小的。IPLH分別啟動ADH和ALDH42%和29.2%，高於IPIVLL。

結果 (繁體中文) 3:

本研究旨在尋找一種從蘑菇足中選取生物活性肽的有效方法，並測定其對體外乙醇代謝酶活性的影響。在壓力為400mpa，處理時間為10min的條件下，以超高壓法選取蘑菇脚肽為最佳工藝條件，超濾後分子量為0-3kda的肽對乙醇脫氫酶（ADH）和醛脫氫酶（ALDH）的活性最高，分別為70.79%和70.79%
分別為71.35%。右旋糖酐凝膠層析後出現兩個峰（p-I和p-II），p-I對ADH和ALDH的活化率分別為72.00%和73.43%，均高於p-II。液相色譜-質譜聯用（LC-MS/MS）測定p-I中有9種肽，其中2種（IPLH和IPIVLL）為合成肽。IPLH對ADH和ALDH的啟動率分別為42.7%和29.2%，均高於IPIVLL。