Fabrication of electrospun thermopl

Fabrication of electrospun thermoplastic polyurethane blended poly (l-lactide-co-e-caprolactone) microyarn scaffolds for engineering of female pelvic-floor tissue.
Potential scaffolds for repair of the female pelvic floor require new materials and fabrication by novel methods to improve cellular infiltration. An 'ideal' engineered scaffold for pelvic-floor tissue should mimic the three-dimensional (3D) network of the extracellular matrix (ECM), which possesses intricate macro- and nano-architecture. In this study, a series of blended poly(l-lactide-co-ecaprolactone) P(LLA-CL)/thermoplastic polyurethane (TPU) microyarn/microfibrous scaffolds were produced with different weight ratios via dynamic liquid electrospinning and electrospinning. Both biopolymers were dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP). Our data showed the mean diameter of microyarn scaffolds to be significantly larger than that of microfibers. Microyarn scaffolds possessed large pore sizes and high porosity. There was no significant difference between the mechanical properties of microyarn and microfibrous scaffolds. Fourier-transform infrared spectroscopy suggested that intermolecular bonds were not present between the molecules of TPU and P(LLA-CL). Morphologic observations using scanning electron microscopy and inverted fluorescence microscopy showed that adipose-derived stem cells labeled with enhanced green fluorescent protein could grow well along or within blend microyarns and migrate within the novel 3D scaffolds. Hematoxylin and eosin staining demonstrated that cell infiltration on microyarn scaffolds was significantly enhanced. The CCK-8 assay showed that microyarns could significantly facilitate cell proliferation compared with microfibrous scaffolds. These results suggested that blend microyarns of P(LLA-CL)/TPU designed to mimic the ECM for female pelvic-floor tissue may be excellent macroporous scaffolds for tissue repair.

結果 (中文) 1:

静电纺丝热塑性聚氨酯的制备共混聚 (l-丙交酯-co-e-己内酯) microyarn 女性盆底组织工程支架材料。女性盆底修复潜在支架需要新材料和制作新颖的方法改善细胞浸润。'理想' 工程支架为盆底组织应该模仿细胞外基质 (ECM)，具有复杂的宏观体系和纳米结构的三维 (3D) 网络。在此研究中，包括与不同重量比通过动态液体静电纺丝技术制作的一系列混合的 poly(l-lactide-co-ecaprolactone) P(LLA-CL)/热塑性聚氨酯 (TPU) microyarn/microfibrous 支架和静电纺丝。这两种生物大分子被溶化在 1,1,1,3,3,3-六氟-2-丙醇 (HFIP)。我们的数据显示 microyarn 支架要明显大于超细纤维平均的直径。Microyarn 支架拥有大孔径、 高孔隙率。有了 microyarn 和 microfibrous 支架的力学性能无显著差异。傅里叶变换红外光谱表明，分子间的债券不存在的 TPU 分子和 P(LLA-CL) 之间。使用扫描电子显微镜和倒置荧光显微镜观察表明增强型绿色荧光蛋白标记的脂肪源性干细胞可以生长好沿或内混合 microyarns 和迁移在新型三维支架内的形态学观察。苏木精和伊红染色显示，microyarn 支架上的细胞浸润明显增强。CCK-8 测定表明 microyarns 可极大地促进细胞增殖与 microfibrous 支架相比。这些结果提示的 P(LLA-CL)/TPU 共混 microyarns 旨在模拟 ECM，因为女性盆底组织可能有优秀的大孔支架组织修复。

結果 (中文) 3:

热塑性聚氨酯制备静电纺混纺聚（l-lactide-co-e-caprolactone）microyarn支架用于女性盆底组织工程。
对女性盆底修复潜在的支架需要新的材料和新的制作方法来提高细胞浸润。一种“理想”的盆底组织工程支架应该模仿的三维（三维）网络的细胞外基质（细胞外基质），具有复杂的宏观和纳米结构。在这项研究中，一系列的混合聚（l-lactide-co-ecaprolactone）P（LLA-CL）/热塑性聚氨酯（TPU）microyarn /微纤维支架进行了不同比例的动态液体静电纺丝法和静电纺丝生产经。这两种生物聚合物溶解在以六氟异丙醇（HFIP）。我们的数据显示，microyarn支架平均直径要明显大于纤维。microyarn支架具有大孔径和孔隙率高。没有显著差异microyarn和微纤维支架的力学性能。傅里叶变换红外光谱表明，分子间的键不存在分子间的TPU和P（LLA-CL）。形态学观察使用扫描电子显微镜和倒置荧光显微镜观察表明，脂肪干细胞标记的增强型绿色荧光蛋白也能生长在或在混合microyarns和迁移在新型三维支架。苏木精-伊红染色显示细胞浸润在microyarn支架显著增强。CCK-8检测发现microyarns能显著促进细胞增殖和微纤维支架相比。这些结果表明，P（LLA-CL）/聚氨酯共混microyarns设计模仿女性盆底组织ECM可以修复组织优良的大孔支架。