Cells were grown on 13 mm Thermanox™ coverslips (catalog
number 26028, Thermanox, Nunc, Rochester, NY, USA) and
then treated for 24 hours with a corresponding concentration
of Fe@Au (ie, 10 µg/mL Fe@Au for OECM1 or 50 µg/mL
Fe@Au for CRC). Cells were then fixed with 2% glutaraldehyde
(catalog number G5882, Sigma-Aldrich) for 1 hour
at 4°C. Samples were washed twice in PBS, followed by
secondary fixation in 1% osmium tetroxide in PBS for 1 hour
at 4°C, in the absence of light. Subsequently, samples were
dehydrated in graded ethanol solutions. Cells were soaked in
30%, 50%, 70%, 80%, 90%, and 95% ethanol for 5 minutes
Cells were grown on 13 mm Thermanox™ coverslips (catalognumber 26028, Thermanox, Nunc, Rochester, NY, USA) andthen treated for 24 hours with a corresponding concentrationof Fe@Au (ie, 10 µg/mL Fe@Au for OECM1 or 50 µg/mLFe@Au for CRC). Cells were then fixed with 2% glutaraldehyde(catalog number G5882, Sigma-Aldrich) for 1 hourat 4°C. Samples were washed twice in PBS, followed bysecondary fixation in 1% osmium tetroxide in PBS for 1 hourat 4°C, in the absence of light. Subsequently, samples weredehydrated in graded ethanol solutions. Cells were soaked in30%, 50%, 70%, 80%, 90%, and 95% ethanol for 5 minutes
正在翻譯中..
![](//zhcntimg.ilovetranslation.com/pic/loading_3.gif?v=b9814dd30c1d7c59_8619)