Twenty-four healthy, homogeneous Saanen goats (mean ± SD; BW of 55.6 ± 1.3 kg, BCS of 3.5 ± 1.0, lactating and not pregnant) were used to conduct this experiment. Experimental goats were randomly distributed according to the previous number of parturitions, milk yield, BW, and BCS. These 24 goats were subjected to 6 treatments: first milking, vaccination, vermifugation, preventive hoof trimming, and placebo (saline solution) or ACTH administration. The ACTH dose tested (0.6 UI of ACTH/kg of BW per animal) was based on previous studies that demonstrated that cortisol returned to basal levels just 6 h after ACTH administration (Fulkerson and Jamieson, 1982; Alam et al., 1986; Schwinn et al., 2016). This cortisol profile was considered similar to other acute stressors related to farm management (Fulkerson and Jamieson, 1982; Negrao et al., 2004; Negrao, 2008)