Reverse zymography is a sensitive method which can detect
several inhibitors at the same time, both characterized inhibitors such as the TIMPs as well as novel ones. If a fluorescently labeled substrate is used, the substrate degradation can
be followed “real-time” and analyses will not suffer from artifacts that may be produced by protein staining [34, 35].Therefore, to be able to determine enzyme activity in a tissue,
several methods should ideally be combined.