In general in the four groups, there was continuous weight gain, but animals fed the H diet showed a higher weight gain, due to the high fat content in the diet. Hence, animals of HA and HL groups could be considered obese since their final body weight was 30 % more than that of either of the other groups, with a body increase significantly higher. In addition, in this study, we chose a nutritional model that has been used previously by other authors [26, 27] in which the H diet leads to steatosis, which can be confirmed by plasma levels of ALT and AST, presence of dyslipidemia (Table 3), liver histological examination (Fig. 1) and assessment of total liver fat (Table 3).