Several studies have tested the effects of astaxanthin or sesame lignan separately on fatigue. Hongo et al., reported that astaxanthin reduced daily fatigue stemming from mental and physical causes [17]. Takemoto et al., evaluated the effects of supplements including sesame lignans and vitamin E on subjective outcomes such as fatigue, sleep and physical appearance [18]. However, the combination of these two components has never been studied for anti-fatigue activity. The objective of this study is to investigate the effect of a mixed supplement containing astaxanthin and sesamin (AS) on daily fatigue. We evaluated subjective fatigue using markers of daily fatigue (visual analog scale (VAS) for fatigue and the Chalder fatigue questionnaire (CFQ)).