Abstract Chitinase specific activity

Abstract Chitinase specific activity was measured spectrophotometrically in wheat leaf tissues during the compatible and incompatible interactions with Puccinia striiformis f. sp. tritici, the causal agent of yellow rust disease. The wheat cultivar, Federation∗ 4/Kavkaz, was inoculated with virulent (134E134A+) or avirulent (4EOA+) races of P. striiformis f. sp. tritici in the first leaf stage. The results showed that chitinase activity pattern was similar in both compatible and incompatible interactions up to 72 hrs after inoculation. However, the specific activity increased rapidly in the incompatible reaction thereafter. In susceptible reaction, chitinase activity gradually declined after 72 hrs post-inoculation reaching a level similar to that in the control plants two weeks after inoculation. Chitinase specificactivityinresistanceresponsewasatleastthreetimesgreaterthanthatinthesusceptiblereactiontwoweeks followingthe inoculation.Electrophoresisof nativepolyacrylamidegel impregnatedwith 0.1% (w/v)glycolchitin as the substraterevealedthe presenceofeightchitinaseisoformswith relativeelectrophoreticmobility(Rm)values ranging from 0.11 to 0.64 in the resolving gel.
Key words: chitinase, Puccinia striiformis f. sp. tritici, resistance, wheat; yellow rust disease

結果 (中文) 1:

抽象的几丁质酶具体活动条锈病锈，黄锈病病原的兼容和不兼容交往中小麦叶片组织内分光光度法测定。小麦品种，Federation∗ 4/高加索，在第一批叶期接种了剧毒 （134E134A +） 或无毒 （4EOA +） 的锈叶部的种族。结果表明，这种几丁质酶活性模式是类似的兼容和不兼容的相互作用，长达 72 小时后接种。然而，具体活动迅速增加在此后不相容的反应。在敏感的反应，几丁质酶活性逐渐下降 72 小时后接种达到类似于控制植物接种后的两个星期后。几丁质酶 specificactivityinresistanceresponsewasatleastthreetimesgreaterthanthatinthesusceptiblereactiontwoweeks 参考接种。Electrophoresisof nativepolyacrylamidegel impregnatedwith substraterevealedthe presenceofeightchitinaseisoformswith relativeelectrophoreticmobility (Rm) 值从 0.11 到 0.64 解决凝胶中作为 0.1%(w/v) glycolchitin。关键词︰ 小麦条锈菌几丁质酶叶部、 抗小麦;黄锈病

結果 (中文) 2:

摘要几丁质酶特定网络C的活性是在与小麦条F中的兼容和不兼容的相互作用分光光度计测量小麦叶片组织。SP。小麦黄锈病的致病因子。在小麦品种，联邦* 4 /高加索，用有毒力（134E134A +）或条锈菌的F无毒（4EOA +）比赛接种。SP。小麦中的第一个叶期。结果表明，几丁质酶活图案在两种相容和不相容的相互作用达到接种后72小时相似。然而，特定网络C的活性在不兼容的反应迅速增加之后。在易感反应，几丁质酶活逐渐72小时后接种后到达在对照植物接种两周后相似的水平下降。几丁质酶特定网络连接cactivityinresistanceresponsewasatleastthreetimesgreaterthanthatinthesusceptiblereactiontwoweeks followingthe inoculation.Electrophoresisof nativepolyacrylamidegel impregnatedwith 0.1％（重量/体积）glycolchitin作为substraterevealedthe presenceofeightchitinaseisoformswith relativeelectrophoreticmobility（RM）值范围为0.11至在分离胶0.64。
关键词：几丁质酶，小麦条F。SP。小麦，电阻，小麦; 黄锈病 小麦条F。SP。小麦，电阻，小麦; 黄锈病 小麦条F。SP。小麦，电阻，小麦; 黄锈病

結果 (中文) 3:

分光光度法测定小麦叶片组织相容和不相容的小麦条锈菌互作过程中特定的抽象fiC几丁质酶活性，条锈病病原。小麦品种，联邦∗4 / Kavkaz，接种强毒（134e134a +）或弱（4eoa +）P.条锈菌在第一阶段比赛fi叶。结果表明，几丁质酶活性模式的兼容和不兼容的相互作用达72小时后接种是相似的。然而，特定的fiC活性迅速上升在不亲和反应之后。在敏感的反应，72小时后接种达到相似的水平，在控制植物两周后接种后几丁质酶的活性逐渐下降。具体ficactivityinresistanceresponsewasatleastthreetimesgreaterthanthatinthesusceptiblereactiontwoweeks几丁质酶在接种。电泳nativepolyacrylamidegel、浸渍0.1%（W/V）截留率为substraterevealedthe presenceofeightchitinaseisoformswith relativeelectrophoreticmobility（RM）值的范围从0.11到0.64的分离胶。关键词：几丁质酶，小麦条锈菌，抗锈病，小麦；