The antifungal effects of Lactobaci

The antifungal effects of Lactobacillus casei AST18 on Penicillium chrysogenum. were investigated. Viable
bacteria were used to detect the minimum inhibitory concentration of lactic acid bacteria (10
2
CFU/m L)
to inhibit the growth of P. chrysogenum. Results showed that L. casei AST18 inhibited the growth of
P. chrysogenum. The mycelial morphology was also altered. The scanning electron microscopy images of
P. chrysogenum exposed to L. casei AST18 further showed that the mycelia were collapsed and appeared
transparent. Numerous clots were also observed in the fungal cytoplasm. The tip of the hypha was
deformed and exhibited a moniliform or in
fl
ated shape. The transmission electron microscopy images of
L. casei AST18-exposed P. chrysogenum showed the occurrence of autophagy and death of fungal cells.
Changes in the morphological characteristics of P. chrysogenum were observed in the antifungal activity
experiment with viable bacteria, but such changes were not evident in the cell-free culture
fi
ltrate of
L. casei AST18. This bacterial strain showed high antifungal activities and could be used as a fungal in-
hibitor in food and medicine.

結果 (中文) 1:

对产黄青霉干酪乳杆菌 AST18 的抗真菌药物影响。进行了研究。可行细菌被用来检测的最低抑菌浓度的乳酸细菌 (102CFU/m L)能抑制 P.霉生长。结果表明: 杆菌 AST18 生长抑制作用P.霉。菌丝形态也有了变动。扫描电子显微镜图像的P.霉暴露 L.干酪乳杆菌 AST18 进一步表明，菌丝体崩溃出现透明。众多的凝块也观察真菌的细胞质中。菌丝的提示变形和展出念珠状或在£ ¬ 也不等式的形状。透射电子显微镜图像的L.干酪乳杆菌 AST18 暴露点霉表明细胞自噬的发生和真菌细胞的死亡。P.霉的形态特征观察中抗真菌活性试验菌活菌，但是这种变化并不明显在无细胞的培养文件ltrateL.干酪乳杆菌 AST18。此菌株表现出高的抗真菌活性和可以用作真菌在-独联体食品和药品。

結果 (中文) 3:

干酪乳杆菌ast18对产黄青霉的抑菌作用。进行了调查。可行的
菌进行乳酸菌的最低抑菌浓度（10
2
CFU / L）
抑制产黄青霉生长。结果表明，干酪乳杆菌ast18抑制
产黄青霉生长。菌丝形态也发生了改变。扫描电子显微镜图像的
产黄青霉在干酪乳杆菌ast18进一步表明菌丝体倒塌，出现
透明。真菌细胞质中也观察到大量的凝块。菌丝的尖端
变形表现出念珠状或
fl
了形状。透射电子显微镜图像的
干酪乳杆菌ast18暴露P.产黄青霉表明自噬与真菌细胞死亡的发生。
是活菌的抑菌活性
实验中观察到的产黄青霉的形态特征的变化，但这种变化并不明显，在无细胞的培养
fi
滤液的
干酪乳杆菌ast18。这种细菌菌株表现出较高的抗真菌活性，并可作为真菌在-在食品和药物制剂。