Negative carry-over effects on milk yield were observed only at the M2 and M3 milkings and for treatments 40 and 0% (Table 1). Compared with the 100% treatment, cows showed or tended to show a change in production of −1.0 kg (P < 0.08) and −1.5 kg (P < 0.05) at the M2 milking (i.e., −5 and −6.8%) and −1.0 kg (P < 0.09) and −1.3 kg (P < 0.03) at the M3 milking (i.e., −6.6 and −8.6%; SED = 0.58 kg) for the 40 and 0% treatments, respectively.