To assess the therapeutic effects o

To assess the therapeutic effects of the human umbilical cord blood (hUCB) derived mesenchymal stem cells (MSCs) on rat bone marrow (BM) exposed to gamma rays, 3 groups (n=15 each) of adult male Wistar albino rats were utilized as follows: the 1st group received PBS (control group), the 2nd group was exposed to gamma rays 1.04Gy/min (R group) and the 3rd group exposed to same dose as RG and injected hUCB-MSCs. The BM of femurs was processed for histological and immunohistochemical staining with proliferating cell nuclear antigen antibody (PCNA), anti human CD105 and anti human CD34. Hb content, leukocytes and platelet counts were analyzed as well as fat cells and megakaryocytic counts. Also, the BM vascular spaces and the optical density of immunostaining for PCNA were analyzed. The leukocytes and platelet counts were significantly lower in the R (2.85±235.8; P=0.000 and 95.27±3.01; P=0.000 respectively) when compared with the control (10.40±443.2; P=0.000 and 430.18±20.28; P=0.000 respectively). The fat cell count was significantly higher in the R (36.55±1.83; P=0.000) than in control (7.64±0.61; P=0.000) and in R injected h-MSCs tissues (18.82±2.03; P=0.000). The megakaryocytic count was significantly higher in the R injected h-MSCs (5.36±0.310; P=0.000) than in control (2.82±0.263; P=0.000) and in the R BM (0.45±0.157; P=0.000). The vascular spaces were dilated and significantly increased in the R injected h-MSCs (50.10±2.40; P=0.000) than in control (33.36±1.01; P=0.000). The optical density of PCNA expression was significantly lower in R (0.18±0.11; P=0.005) than in control (0.41±0.40; P=0.005) and in R injected h-MSCs groups (0.30±0.17; P=0.005). The present study concluded that injection of hUCB-MSCs improves destructive effects of bone marrow induced by gamma radiation. Use of radio-protective agents during exposure is recommended.

結果 (中文) 1:

为评估疗效的人脐带血 （脐血） 推导间充质干细胞 (MSCs) 对大鼠骨髓 (BM) 暴露在伽玛射线，3 组 (n = 15 每个) 的成年雄性 Wistar 大白鼠的使用情况如下︰ 第一批收到 PBS （对照组），第二集团被暴露了伽玛射线 1.04Gy / min （R 组） 和第三组暴露于同一剂量作为 RG 和注入脐血间充质干细胞。BM 的股骨被处理为组织学和免疫组化染色与增殖细胞抗体核抗原 (PCNA) 人类 CD105 和抗人脐血 CD34。血红蛋白含量、 白细胞和血小板计数进行分析以及脂肪细胞和巨核细胞计数。同时，分析了 BM 血管空格和增殖细胞核抗原蛋白免疫的光学密度。白细胞和血小板计数均显著低于 R (2.85±235.8;P = 0.000 和 95.27±3.01;P = 0.000 分别) 与对照 (10.40±443.2;P = 0.000 和 430.18±20.28;P = 0.000 分别)。脂肪细胞计数均显著高于 R (36.55±1.83;P = 0.000) 比在控制 (7.64±0.61;P = 0.000) 和在研发注入 h MSCs 组织 (18.82±2.03;P = 0.000)。巨核细胞计数均显著高于注射 R h-间充质干细胞 (5.36±0.310;P = 0.000) 比在控制 (2.82±0.263;P = 0.000) 和 R BM (0.45±0.157;P = 0.000)。扩张血管的空间并显著增加注射的 R h-间充质干细胞 (50.10±2.40;P = 0.000) 比在控制 (33.36±1.01;P = 0.000)。增殖细胞核抗原表达的光学密度低于 R (0.18±0.11;P = 0.005) 比在控制 (0.41±0.40;P = 0.005) 和在研发注入 h MSCs 组 (0.30±0.17;P = 0.005)。本研究的结论脐血间充质干细胞注射液改善骨髓伽玛辐射诱导的破坏性影响。建议在曝光期间的辐射防护剂的使用。

結果 (中文) 2:

为了评估成年雄性Wistar大白鼠的人脐带间充质干细胞（MSCs）对大鼠骨髓（BM）暴露在伽马射线，3组血（人脐血）的治疗效果（15只）被用作如下：第1组接受PBS（对照组），第二组暴露于伽马射线1.04Gy /分钟（R组）和第三组暴露于相同剂量的RG和注射人脐血间充质干。股骨的骨髓进行处理与增殖细胞核抗原抗体，组织学及免疫组化染色（PCNA），抗人CD105和抗人CD34。血红蛋白含量，白细胞和血小板计数进行分析，以及脂肪细胞和巨核细胞计数。此外，在BM血管空间和免疫染色对PCNA的光密度进行分析。白细胞和血小板计数均在R显著低（2.85±235.8; P = 0.000和95.27±3.01; P = 0.000分别）时，与对照（10.40±443.2相比，P = 0.000和430.18±20.28; P = 0.000分别）。脂肪细胞计数在R显著较高（36.55±1.83; P = 0.000）比对照组（7.64±0.61; P = 0.000），并在注射了R H-干细胞组织（18.82±2.03; P = 0.000）。的巨核细胞数是在R显著更高注射H-的MSCs（5.36±0.310; P = 0.000）比对照（2.82±0.263; P = 0.000）和在R BM（0.45±0.157; P = 0.000）。血管空间进行扩张，在R显著上升注入H-干细胞（50.10±2.40; P = 0.000）比对照组（33.36±1.01; P = 0.000）。PCNA的表达的光密度中的R是显著低（0.18±0.11; P = 0.005）比对照（0.41±0.40; P = 0。005）和R中注入H-的MSC组（0.30±0.17; P = 0.005）。本研究得出的结论是人脐血间充质干注入提高了γ射线引起的骨髓的破坏作用。建议曝光期间无线电保护剂的用途。

結果 (中文) 3:

探讨人脐带血的疗效（脐血）间充质干细胞（MSCs）对大鼠骨髓（BM）暴露于γ射线，3组（n = 15）的成年雄性Wistar大白鼠使用如下：第一组接受PBS（对照组），第二组暴露于γ射线1.04gy/min（R组）和第三组暴露于相同剂量的RG和注入hUCB MSCs。股骨骨髓进行组织学和免疫组织化学与细胞增殖核抗原（PCNA）抗体染色，抗人CD105和抗人CD34。血红蛋白含量、白细胞和血小板计数分析，以及脂肪细胞和巨核细胞计数。此外，骨髓血管空间和PCNA的光密度分析。白细胞和血小板计数在R（2.85＝235.8；P = 0和95.27×3.01；P = 0），与对照组相比显着降低（10.40 = 443.2；P = 0和P分别为）。脂肪细胞数显著高于R（36.55±1.83；P = 0）比对照组（7.64±0.61；P = 0）和R（18.82±注入h-mscs组织2.03；P = 0）。巨核细胞计数在R显著高于注射h-mscs（5.36±0.310；P = 0）比对照组（2.82±0.263；P = 0）和R BM（0.45±0.157；P = 0）。血管腔扩张和R显著增加注入h-mscs（50.10±2.40；P = 0）比对照组（33.36±1.01；P = 0）。R中的PCNA表达的光密度明显降低（0.18±0.11；P = 0.005）比对照组（0.41±0.40；P = 0.005）和R（0.30±注入h-mscs组0.17；P = 0.005）。目前的研究表明脐血间充质干细胞注射来提高γ辐射诱导骨髓破坏作用。建议在暴露期间使用无线电防护剂。