The relative configurations around

The relative configurations around the bis-THF rings were determined by analysis of the 1H NMR spectrum and
comparison with literature data. In Figure S3a, the chemical shifts atδH 3.39 (H-21) and 3.84 (H-20) indicated the S* and S* relative configurations, while the S* and R* relative configurations were deduced for C-12 and C-13 and for C-16 and C-17, due to the similarity of the chemical shift of H-12 (δH3.87) and H-13 (δH3.83) and that of H-16 (δH3.93) and H-17 (δH3.91).17,18The methylene protons H-18 and H-19 were assigned to signals at δH1.60 and 1.97 on the basis of COSY correlations between H-20 and H-21, H-19 and H-20,and H-18 and H-17, combined with HMBC coupling between H-19 and C-17. The difference between these chemical shifts indicated a trans configuration for the C-17/C-20 THF ring.19In the same manner, a trans configuration was assigned to the C-13/C-16 THF ring based on the divergent chemical shifts atδH1.62 and 1.87 for H-14 and H-15.19Thus, the relative configuration around the bis-THF rings was concluded to be S*, R*, R*, S*, S*, S* for C-12, C-13, C-16, C-17, C-20, and C-21. The absolute configuration of C-36 was determined by CD spectroscopic analysis. The negative n−π* Cotton effect (Δε =−1.13) at 238 nm and a positive π−π* Cotton effect (Δε =8.79) at 208 nm clearly indicated the S configuration at C-36 in the lactone ring.16The absolute configurations of the OH groups adjacent to the THF rings were determined by Mosher ester methodology20 using the (R)-MPA and (S)-MPA derivatives21 and the “in NMR tube” method.22As shown in Figure S4a, the ΔδH(=δS− δR) was negative on both chain sides and positive along the THF rings, indicating S configurations at both C-12 and C-21.23The configuration of C-8 could not be determined due to the negative ΔδH values displayed by both H-7 and H-9 on the hydrocarbon chain.

結果 (中文) 1:

国际清算银行-四氢呋喃环周围的相对配置测定 1h 核磁共振谱分析和与文献数据比较。在图 S3a 的化学位移 atδH 3.39 (H-21) 和 3.84 (H-20) 表明 S * S * 相对配置，而推导出的 S 和 R 的相对配置了 C-12 及 C-13 和 C-16 和 C-17，由于化学位移的 H-12 (δH3.87) 和 H-13 (δH3.83) 的相似性和 H-16 (δH3.93) 和 H-17 (δH3.91).17，18The 亚甲基质子 H-18 和 H-19 时，被分配到信号在 δH1.60 和 1.97 舒适相关性的基础上H-20 H-21、 H-19 和 H-20，H-18 和 H-17，结合 H-19 和 C-17 惠丰耦合。这些化学位移之间的差异表明 C-17/C-20 四氢呋喃 ring.19In 跨配置相同的方式，跨配置被分配到基于发散化学位移 atδH1.62 和 1.87 H-14 和 H C-13/C-16 四氢呋喃环-15.19Thus，四周之二-四氢呋喃环相对配置被定论为 S * R *，R *，S *，S *，S * 为 C-12C-13 C-16 C-17，C-20、、 C-21。C-36 的绝对构型测定 CD 光谱分析。消极的 n−π * 棉效果 (δ ε = −1.13) 在 238 nm 和积极 π−π * 棉效果 (δ ε = 8.79) 208 nm 清楚表明在 C-36 中 OH 基团相邻的四氢呋喃环内酯 ring.16The 绝对构型的 S 配置测定毛思迪酯 methodology20 使用 （R)-MPA 和 (S)-MPA derivatives21 和图 S4a 所示的"在核磁共振管"method.22As的 ΔδH （= δS− δR） 是负链两侧和正面沿四氢呋喃环，该值指示在 C-12 和 C S 配置-21.23The C-8 配置无法确定由于负 ΔδH 显示值由 H-7 和 H-9 烃链上。

結果 (中文) 3:

在双四氢呋喃环的相对位置是由1H NMR光谱分析测定文献资料比较。图S3A，在δH的化学位移（3.39型）和3.84（H-20型）表示S *和*相对配置，而S和R *导出C-12和C-13和C-16和C-17相对配置，由于对12化学位移的相似性（δh3.87）和H-13（δh3.83），软件（δh3.93）和h-17（δh3.91）。17,18the亚甲基质子H和H-19在δh1.60和1.97在舒适型之间的相关性和H-20型的基础上分配给信号，H-19和H-20型，和H和h-17，结合H-19和C-17之间分离耦合。这些化学位移之间的差异表明C-17 / C-20 THF ring.19in相同的方式反式构型，反式构型的C-13 / C-16四氢呋喃环以1.87δh1.62 H-14和h-15.19thus发散的化学位移，在双四氢呋喃环的相对配置的结论是，R *，*，*，*，为C-12、C-13、C-16，C-17，C-20的*，和C-21。通过CD光谱分析测定条件的绝对构型。负氮−π棉花效应（Δε=−1.13）在238 nm和积极的π−π棉花效应（Δε= 8.79）清楚地表明在208 nm的配置在相邻的羟基四氢呋喃环内酯ring.16the测定绝对构型C-36 Mosher酯methodology20使用（R）MPA和（S）- MPa derivatives21和“核磁管”method.22as图S4a所示，的ΔδH（=δ的−δR）为负两链两侧和正沿着四氢呋喃环的配置，说明在C-12和C-8 c-21.23the配置无法在H-7和H-9在烃链显示负ΔδH值确定。