Abstract: The optical properties of fruits, such as light absorption and scattering characteristics, change with biochemical activities during storage. Diffuse reflectance spectroscopy (DRS) systems have been widely applied for noninvasively observing biological tissues. In this study, we used a frequency-domain DRS system to measure the optical properties of apples. Results showed that variationsinthechlorophyll,water,andflesh-textureofapplescouldbenoninvasivelymonitoredover time. We also observed substantial differences in the absorption and reduced scattering coefficients between injured and normal apples. The DRS techniques could be used for apple grading, and, by extension, for monitoring the quality of other fruits.