Other situations needing extra care to prevent unwanted mutations might require an enzyme with proofreading activity. If you need high yields of quality DNA for applications such as microarrays or NGS, a high-processivity enzyme with fast extension times may be your choice, such as EMD Millipore’s high-fidelity KOD DNA Polymerase, or the Thermo Scientific Phusion High-Fidelity DNA Polymerase. If the PCR reaction assembly is more convenient at room temperature, such as to use automated robotic systems, or if specificity is especially challenging, look for a hot-start version of the DNA polymerase.