Abstract In this study, we develope

Abstract In this study, we developed tissue culture protocol of Salvia dolomitica, a particularly fragrant sage and
we evaluated the yield and the profile of essential oil (EO)
produced from plants grown in vivo compared to in vitro
plant material cultured in two light intensity growth conditions. Plant extracts secreted from glandular trichomes are, in
Lamiacea, a valuable source of biologically active volatile
compounds. Microscopical observations permitted to
describe, for the first time, secretory structures fromS. dolomitica leaves developed in vitro showing morphological
identity to in vivo glands. The yield of EO obtained from
light-stressed in vitro plants was higher than which obtained
from the non-stressed in vitro plants and threefold higher that
in vivo plants. Phytochemical analysis of EOs and volatile
compounds showed quali-quantitative differences between
S. dolomiticain vitro grown plantlets and those from open
field.a-Pinene, b-phellandrene and borneol accumulated
predominantly in micropropagated plants whileb-caryophyllene

結果 (中文) 1:

在本研究中，我们开发了丹参 dolomitica，尤其是香的鼠尾草组织培养协议和我们评估了产量和精油 （EO） 概况产生的植物生长在体内与体外相比植物材料体外培养两个光强生长条件。植物提取物由具腺毛状体分泌出来的是在Lamiacea，宝贵的具有生物活性的挥发性化合物。允许的显微观察第一次描述分泌结构即是正书。dolomitica 叶铁流体外形态体内腺体的身份。所得的环氧乙烷收率光强调体外植物明显高于得到从非强调在体外植物及三倍以上，在植物体内。EOs 及挥发性化学成分分析化合物显示质量定量差异S.dolomiticain 体外生长植株和那些从开放field.a-蒎烯、 b 水芹和冰片积累主要是在试管中植物 whileb 石竹烯

結果 (中文) 3:

摘要：在这项研究中，我们开发了丹参dolomitica组织培养协议，特别香，鼠尾草和
我们评估产量和精油（EO）
轮廓产生在体内生长的植物相比，体外培养的两
植物材料的光强度增长条件。植物提取物，腺毛分泌，在
lamiacea，一个有价值的生物活性化合物的挥发性
源。显微镜观察允许
描述，为第一次，分泌结构形式。dolomitica叶体外形态
身份体内腺。EO从
光获得的产量强调体外植株高于得到
从非预应力体外植物和三倍高于
体内的植物。EOS和挥发性化合物的化学分析表明
quali-quantitative
dolomiticain之间在体外生长的植株和开放式
field.a-pinene b-phellandrene差异，冰片积累主要在石竹试管植物whileB