The method of laser needle acupuncture has been described in detail in the first and second parts of the study and in previous work. Noninvasive violet optical needles (wavelength, 405 nm; output power, 110 mW; laser needle spot diameter, 500 mm; time, 10 min) were fixed onto but not inserted into the skin. The same research system from Laserneedle EG GmbH (Wehrden, Germany) as mentioned in parts 1 and 2 of the study was used for the measurements. This equipment consists of ten semiconductor injection laser diodes. Each single needle can emit at a different wavelength. In the present study, a continuous wave (CW) mode was used. Due to coupling losses, the output at the tip of the laser needle is approximately 100 mW. Irradiation lasted 10 min (600 seconds), and therefore optical power energy density was very high (in kJ/cm2 range).