Although the detailed processes of scaling up the
procedures involved in structure determination are
unique to each centre for structural genomics, several
characteristics are shared among most centres (FIG. 2).
The experimental process begins with the cloning of
selected target sequences, frequently with recombination-
based vectors that allow the creation of many different
constructs. These vectors incorporate different
affinity tags, such as HIS-TAGS and glutathione-S-transferase
(GST), to aid purification, as well as promoters