Figure 6. Alanine/Glycine-Scanning

Figure 6. Alanine/Glycine-Scanning Mutagenesis Experiments on NPC1-C
(A–I) BIAcore diagrams of quintuple-G, Y423G&P424G, F503G&F504G, F503A&F504A, F503G, F504G, F503A, F504A, and Y506A mutants of NPC1-C binding to
the GPcl. These nine mutants abolish the binding to the GPcl.
(J) BIAcore diagram and saturation curve of P424G mutant of NPC1-C binding to the GPcl. The P424G mutant can keep binding to the GPcl with an affinity of
213 mM, which is slightly lower than that of wild-type NPC1-C.
(K) BIAcore diagram and saturation curve of Y423A mutant of NPC1-C binding to the GPcl. The Y423A mutant can keep binding to the GPcl with an affinity of
166 mM, which is slightly lower than that of wild-type NPC1-C.
(L) BIAcore diagram and saturation curve of P424A mutant of NPC1-C binding to the GPcl. The P424A mutant can keep binding to the GPcl with an affinity of
281 mM, which is slightly lower than that of wild-type NPC1-C. Response units were plotted against protein concentrations. The KD values were calculated by the
BIAcore 3000 analysis software (BIAevaluation Version 4.1).
See also Figures S6 and S7.

結果 (中文) 1:

图 6。NPC1 C 丙氨酸甘氨酸层扫描诱变试验(A — — 我)五冠 G BIAcore 图、 Y423G 及 P424G、 F503G 及 F504G、 F503A & F504A、 F503G、 F504G、 F503A、 F504A 和 Y506A 突变体 NPC1 C 绑定到罗辉。这些九个突变体取消绑定到和平共处。（J) BIAcore 图和饱和曲线 P424G 突变体 NPC1 C 绑定到和平共处。P424G 突变体可以保持绑定到具有亲和力的罗辉213 毫米，是略低于野生型 NPC1 C.（K) BIAcore 图和饱和曲线 Y423A 突变体 NPC1 C 绑定到和平共处。Y423A 突变体可以保持绑定到具有亲和力的罗辉166 毫米，是略低于野生型 NPC1 C.（L) BIAcore 图和饱和曲线 P424A 突变体 NPC1 C 绑定到和平共处。P424A 突变体可以保持绑定到具有亲和力的罗辉281 毫米，是略低于野生型 NPC1 C.响应单元被密谋反对蛋白浓度。KD 值计算了BIAcore 3000 分析软件 (BIAevaluation 版本 4.1)。另请参阅数字 S6 和 S7。

結果 (中文) 3:

图6。丙氨酸和甘氨酸突变扫描实验npc1-c（一–我）的quintuple-g Biacore图，y423g和p424g，f503g和f504g，f503a和f504a，f503g，f504g，f503a，f504a，和npc1-c结合y506a突变体GPCL。这九个突变废除GPCL结合。（J）和npc1-c Biacore图的结合p424g突变体GPCL饱和曲线。的p424g突变体可以结合亲和力的民法通则213毫米，这是略低于野生型npc1-c.（k）Biacore图和npc1-c突变体的结合y423a GPCL饱和曲线。的y423a突变体可以结合亲和力的民法通则166毫米，这是略低于野生型npc1-c.（l）Biacore图和npc1-c突变体的结合p424a GPCL饱和曲线。的p424a突变体可以结合亲和力的民法通则281毫米，这是略低于野生型npc1-c.响应单元绘制蛋白浓度。计算得到的KD值Biacore 3000分析软件（biaevaluation版本4.1）。参见图S6和S7。