本文主要从硬件设计和软件设计两方面完成对在线尾气分析仪的设计，将设计好

本文主要从硬件设计和软件设计两方面完成对在线尾气分析仪的设计，将设计好的尾气分析仪制成工程样机应用于酿酒酵母发酵生产GSH过程控制，期望达到同时实现发酵尾气采集和过程控制的目的。本论文主要包括以下几个方面的内容：
(1)仪器硬件设计。从核心控制器和传感器的选型到仪器电路、硬件之间的连接完成了仪器硬件电路设计。采用Arduino mega2560单片机作为核心控制器，荧光学氧气传感器和红外二氧化碳传感器作为检测元件，以UART通信协议连接控制器和传感器，大大简化了仪器硬件电路。反馈控制执行模块采用兰格蠕动泵，通过蠕动泵外控模块实现对蠕动泵的控制。
（2）仪器软件设计。下位机程序通过类C语言对串口通信、数据采集、传感器校正、反馈控制等功能模块进行编程，上位机软件利用LabVIEW开发环境编写，采用VISA函数实现对下位机的通信，实现了数据采集与处理、实时显示与存储在内的多个模块的功能，界面直观，运行可靠。整个仪器通过软硬件综合调试运行良好，对比市售尾气分析仪结果显示，本仪器数据测量准确、可靠，能长期稳定运行，达到了预期目标。
（3）呼吸商反馈控制酿酒酵母发酵法生产GSH过程。首先考察了摇瓶发酵、分批发酵、基于指数速率和恒速流加的补料分批发酵这三种发酵方式对酵母细胞生长和GSH合成的影响，发现基于指数速率和恒速流加的补料分批发酵方式细胞密度和GSH合成量较高，发酵培养72小时后细胞干重达到78.22 g/L，GSH产量为1798.48 mg/L。然后将尾气分析仪应用于GSH发酵过程中，采用RQ反馈控制葡萄糖补料速率，经Arduino PID控制将RQ维持在设定值左右。对比不同RQ条件下GSH产量和细胞密度，发现当RQ控制在0.82时GSH和细胞生物量最高，分别达到1.61 g/L 和55.12 g/L（DCW）。添加前体氨基酸能进一步提高细胞GSH产量，在RQ反馈控制发酵过程中添加前体氨基酸，与未添加前体氨基酸相比产量明显提高，GSH总产量达2.47 g/L，提高53.42%，细胞干重达62.18 g/L，提高 12.81%，胞内GSH含量提高了32.27%。

关键词：发酵尾气 呼吸商 谷胱甘肽 Arduino 反馈控制

結果 (中文) 3:

This paper mainly completes the design of online gas analyzer from two aspects of hardware design and software design, exhaust gas analyzer designed into engineering prototype used in the fermentation of Saccharomyces cerevisiae GSH production process control, to realize at the same time fermentation tail gas collection and process control. This paper mainly includes the following aspects: (1) the hardware design of the instrument. From the connection between controller and sensor selection to the instrument hardware circuit, completed the hardware circuit design. Using Arduino mega2560 microcontroller as the core controller, fluorescence, oxygen sensor and infrared carbon dioxide sensor as the detecting element,The communication protocol of the UART is connected to the sensor and controller, which greatly simplifies the hardware circuit. Feedback control execution module using Lange peristaltic pump, through the peristaltic pump control module to realize the control of the peristaltic pump.
(2) instrument software design. The lower computer program by C language programming of serial communication, data acquisition, sensor calibration, the feedback control module, host computer software is programmed with LabVIEW development environment, using VISA function to realize the communication of MCU, realizes the data acquisition and processing, real time display and storage module, function, intuitive interface, reliable operation.The instrument through the software and hardware debugging and running results show that the sale of contrast, exhaust gas analyzer, the instrument measuring data is accurate and reliable, long-term stable operation, to achieve the expected goal.
(3) respiratory quotient feedback GSH control of Saccharomyces cerevisiae fermentation production. First examine the shake flask fermentation, batch fermentation, the exponential rate and a constant flow of feeding and batch fermentation of these three kinds of fermentation effects on growth and GSH synthesis of yeast cells were found based on the exponential rate, and a constant flow of the fed batch fermentation was higher cell density and GSH synthesis based on the fermentation, 72 hours later, cell dry weight to 78.22 g/L,The yield of GSH was 1798.48 mg/L. And then the exhaust gas analyzer was applied to GSH in the fermentation process, the RQ feedback control of glucose feeding rate, the Arduino PID control RQ maintained at the set value. Comparison of different RQ conditions, GSH production and cell density, found that when the RQ control in the 0.82 GSH and the cell biomass was the highest, reached 1.61 g/L and 55.12 g/L respectively (DCW). Adding the precursor amino acid can improve the cell yield of GSH, adding the precursor amino acids in the fermentation process control RQ feedback, compared with not adding the precursor amino acid yield was increased, GSH production reached 2.47 g/L, increased by 53.42%,Cell dry weight of 62.18 g/L, increased by 12.81%, the content of intracellular GSH was increased by 32.27%. Keywords:

fermentation tail gas respiratory quotient of glutathione Arduino feedback control.