AbstractCervical cancer is the prin

Abstract

Cervical cancer is the principal cause of mortality due to cancer in women worldwide. New predictive markers may increase survival rates by improving the treatment of patients at a high risk for cancer. This study was carried out to investigate the amplification of human telomerase RNA component (hTERC) or/and c-MYC in cervical epithelial exfoliated cells for cervical carcinoma screening. We collected 171 specimens. including speciments from normal cervix, benign lesions, cervical intraepithelial neoplasia (CIN)1, CIN2 and CIN3, or carcinoma in situ, as well as invasive cervical squamous cell carcinoma. Fluorescence in situ hybridization (FISH) was performed to detect alterations in hTERC and c-MYC expression. We analyzed the area under the receiver operating characteristic (ROC) curve (AUC), as well as the sensitivity and specificity of single screening and conjoined screening. There was a trend toward an increasing amplification of 2 genes with the increasing severity of cervical lesions. ROC curve analysis demonstrated that the AUC values of the hTERC gene for the screening of different cervical lesions were >0.8. Compared with the hTERC gene, the AUC of the c-MYC gene for the screening of ≥CIN3 was >0.8 and the AUC for the screening of other cervical lesions was >0.7. For the screening of cervical lesions above the grade of benign lesions, cytological diagnosis was superior to the gene detection with significant differences. For the screening of cervical lesions >CIN1, there were no statistically significant differences (P>0.05) between the hTERC gene and cytological diagnosis, whereas the screening results of c-MYC detection and cytological diagnosis differed significantly (PCIN2 or >CIN3, the detection of hTERC and c-MYC genes and cytological diagnosis had similar screening results with no statistically significant differences (P>0.05). In conclusion, using FISH to detect the amplification of hTERC or/and c-MYC on cervical epithelial exfoliated cells may be a useful and specific screening method for precancerous lesions.

結果 (中文) 1:

摘要宫颈癌是全世界妇女的癌症死亡率的主要原因。新的预测指标可能会增加存活率提高癌症高危患者的治疗。这项研究进行了探讨人类端粒酶 RNA 组分 (hTERC) 的放大或 / 和 c-MYC 在宫颈癌筛查宫颈上皮脱落细胞。我们收集 171 标本。包括从正常宫颈良性病变、 宫颈上皮内瘤样病变 (CIN) 1、 CIN2 和 CIN3，或原位癌、 以及创宫颈鳞状细胞癌标本。荧光原位杂交 (FISH) 进行检测中 hTERC 和 C-MYC 表达的改变。我们分析下的接受者操作特性 (ROC) 曲线 (AUC) 地区敏感性和特异性及连体单放映场次。还有 2 基因与日益严重的宫颈病变越来越放大的趋势。ROC 曲线分析表明，hTERC 基因不同宫颈病变筛查的 AUC 值 > 0.8。与 hTERC 基因相比，c-MYC 基因筛选的 ≥CIN3 的 AUC 是 > 0.8 和其他宫颈病变筛查 AUC 是 > 0.7。以上等级的良性病变宫颈病变筛查，细胞学诊断优于基因检测有显著性差异。宫颈病变筛查 > CIN1，无差异有统计学意义 (P > 0.05) hTERC 基因与细胞学诊断而筛选结果的 c-MYC 检测和细胞学诊断差异显著 (P < 0.05)。宫颈病变筛查 > CIN2 或 > CIN3，hTERC 和 c-MYC 基因和细胞学诊断检测有类似筛查结果没有差异具有统计学意义 (P > 0.05)。最后，利用鱼检测扩增的 hTERC 或 / 和 c-MYC 对宫颈上皮脱落细胞可能是癌前病变有用和特定的筛选方法。

結果 (中文) 3:

摘要：宫颈癌是全球妇女癌症死亡率的主要原因。新的预测标志物可以提高患者的生存率，提高治疗癌症的风险。本研究旨在探讨人端粒酶RNA组分的放大（hTERC）或/和C-myc在宫颈上皮脱落细胞宫颈癌筛查。我们收集了171个样本。从正常宫颈标本，良性病变，宫颈上皮内瘤变（CIN）1，CIN2和CIN3，或原位癌，以及浸润性宫颈鳞状细胞癌。荧光原位杂交（FISH）检测hTERC和c-myc基因表达的改变。我们分析了面积受试者工作特征（ROC）曲线（AUC），以及敏感性和特异性，结合单筛选筛选。有一个趋势，越来越多的扩增与宫颈病变的严重程度增加的2个基因。ROC曲线分析表明，不同宫颈病变筛查的hTERC基因的AUC值> 0.8。与hTERC基因相比，对≥CIN3筛选的c-myc基因的AUC为> 0。8，AUC为其他筛查宫颈病变＞0.7。对于宫颈良性病变的筛查，细胞学诊断优于基因检测，具有显著性差异。对宫颈病变的筛查>> CIN1，差异无统计学意义（P＞0.05）hTERC基因及细胞学诊断，而基因检测和细胞学诊断筛查结果差异显著（P＜0.05）。对宫颈病变CIN2或CIN3 > >筛选，hTERC、细胞学诊断c-myc基因的检测也有类似的检测结果差异无统计学意义（P＞0.05）。总之，用FISH检测对宫颈上皮脱落细胞hTERC基因扩增或/和癌前病变的可能是一个有用的和具体的筛选方法。