Control of FMD mainly encompasses vaccination and slaughter policy, particularly for the FMD-free countries, and slaughter policy is considered a fundamental measure. However, some restrictions from economic situation, social culture, geographical and natural environment limit this policy to be applied in a large-scale range in the endemic areas. Consequently, vaccination or the combination policy of vaccination and slaughter remain the most effective countermeasure against FMDV. Though different type of vaccine comprising subunit vaccine, peptide vaccine, DNA vaccine are developed, FMD inactivated vaccine still play a key role in control campaigns and eradication of FMD [55] in the majority FMD epidemic countries and territories because of perfect protection potency. However, another new problem arises from inactivated vaccine of FMD is that it is difficult to distinguish vaccinated from infected animals. And then, A range of ELISA techniques are currently being evaluated with the intention of producing ELISAs for routine diagnostic use which are capable of detecting antibody to FMD virus NSP's. These methods were described as following: