The milk fat concentration and SCS increased at the M1 milking only for the 0% treatment (+1.00 percentage point and +1.2 units, respectively) and increased at the M2 milking for the 40 and 0% treatments (milk fat concentration: +0.63 and +0.79 percentage point, P < 0.02; SCS: +1.2 and +2.2 units, P < 0.0001, respectively; Table 2).