a b s t r a c tPolysaccharides prod

a b s t r a c tPolysaccharides production from the fruiting body of Tricholoma matsutake was carried out using enzymeassisted extraction methodology. Response surface methodology (RSM), based on a five level, four variablecentral composite design (CCD), was employed to obtain the best possible combination of extractiontemperature (X1: 54–66 ◦C), extraction pH (X2: 3.6–4.4), extraction time (X3: 2.4–3.6 h), and complexenzyme (the ratio of papain:pectinase:cellulase was 1:1:1) amount (X4: 1.6–2.4%) for maximum polysaccharidesproduction. The experimental data obtained were fitted to a second-order polynomial equationusing multiple regression analysis and also were analyzed by analysis of variance (ANOVA). The 3-Dresponse surface plot and the contour plot derived from the mathematical models were applied to determinethe optimal conditions. The optimum extraction conditions were: extraction temperature of 61.8 ◦C,extraction pH of 4.14, extraction time of 3.2 h, and complex enzyme amount of 2.1% (w/v). Under theseconditions, the experimental yield of polysaccharides was 7.53±0.26%, which is well in close agreementwith the value predicted by the model

結果 (繁體中文) 1:

抽象
松口蘑的子實體多醣生產進行了使用酶
輔助提取方法。響應面分析法（
中心複合設計（
溫度（- 66 ◦ C - 4.4 - 3.6 h
酶（木瓜蛋白酶的比率：果膠酶：纖維素酶為- 最大多醣
的生產。所獲得的實驗數據擬合至一個二階多項式方程
使用多元回歸分析，並且還通過方差分析（3-d
響應面圖和從所述數學模型獲得的等高線圖被應用到確定
的最佳條件。最佳提取條件為：提取溫度為◦ C
4.14 在這些
條件下，多醣的實驗產量為
±
0.26
與由模型預測的值

結果 (繁體中文) 2:

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結果 (繁體中文) 3:

a b s t r a c t公司
用酶法從鬆口蘑子實體中選取多糖
輔助選取法。回應面方法（RSM），基於五級四變數
採用中心複合設計（CCD）獲得最佳選取組合
溫度（X1:54–66°C）、萃取pH值（X2:3.6–4.4）、萃取時間（X3:2.4–3.6 h）和絡合物
酶（木瓜蛋白酶：果膠酶：纖維素酶的比例為1:1:1）量（X4:1.6–2.4%）最大多糖
生產。所得的實驗數據被擬合成二階多項式方程
採用多元回歸分析，並進行方差分析。三維
應用回應面圖和由數學模型匯出的等高線圖確定
最佳條件。最佳選取條件為：選取溫度61.8℃，
選取pH值4.14，選取時間3.2h，複合酶用量2.1%（w/v）。在這些下麵
實驗條件下，多糖得率為7.53
±
0.26%，非常接近
用模型預測的值