Protective antibodies levels, induced by HBV vaccine, persist forlong-term after primary immunization [6–8], but there is evidencethat, as the time since vaccination increases, there is a reducedability to maintain immune memory [9,10]. Thus, the assessmentof anti-HBs status after immunization is very important in particularwhen subjects are engaged in activities that would put themat high risk of acquiring HBV infection, as in the case of somehealth care workers (HCWs). According to WHO, about 66,000HBV infections per year occur in HCWs worldwide due to bloodborneexposure [11,12]. The risk seems to be higher for healthcarestudents than for employed HCWs, because they are relativelyinexperienced with handling certain instruments and with the executionof specific techniques at risk of accidental blood and bodyfluid exposures [13].