Never again will PCR enzymes be “on

Never again will PCR enzymes be “one-size-fits-all.” Kapa Biosystems takes a directed, high-throughput approach for engineering new DNA polymerases for particular PCR applications, such as NGS. “[Our] ability to screen hundreds of millions of unique proteins allows us to identify enzyme variants with dramatically improved functionality compared to native enzymes,” says John Foskett, technical director at Kapa Biosystems, who engineered the new high-fidelity KAPA HiFi DNA Polymerase for amplifying NGS libraries. “It was optimized to reduce PCR-induced bias and improve yield, resulting in more uniform sequencing coverage and increased library complexity.”

結果 (中文) 1:

PCR酶绝不会再是“一个尺寸适合所有人的。”KAPA生物系统需要导向的，高通量的方法，尤其是PCR技术的应用，如农工商工程新的DNA聚合酶。 “[我们]屏幕数百数以百万计的独特的蛋白质的能力，使我们能够识别的酶变体与天然酶相比，具有显着增强的功能，”约翰说层出不穷，KAPA生物系统，设计新的高精确度KAPA高保真DNA聚合酶扩增农工商库技术总监。 “它被优化，以减少PCR-诱导的偏置，提高成品率，从而导致更均匀的测序覆盖范围和增加图书馆复杂。”

結果 (中文) 2:

Never again will PCR enzymes be "one-size-fits-all." Kapa Biosystems takes a directed, high-throughput approach for engineering new DNA polymerases for particular PCR applications, such as NGS. "[Our] ability to screen hundreds of millions of unique proteins allows us to identify enzyme variants with dramatically improved functionality compared to native enzymes," says John Foskett,在卡帕生物技术总监，曾设计了新的高保真DNA聚合酶扩增卡帕高保真NGS文库。“这是优化PCR诱导减少偏见和提高产量，导致更均匀的序列覆盖和增加图书馆的复杂性。”

結果 (中文) 3:

聚合酶链反应酶再也不会"一刀切"。卡帕生物系统采用的工程，尤其是 pcr 技术的应用程序，如本国一般事务的新 DNA 聚合酶定向、 高吞吐量的方法。"[我们的] 屏幕数以亿计的独特蛋白质的能力让我们认同大大改进功能相比，本机的酶，酶变种"说约翰 · 福斯克特，在卡帕生物系统，那些工程放大个本国一般事务图书馆的新的高保真卡帕 HiFi DNA 聚合的技术总监。「 它优化以减少 PCR 诱导的偏见和提高产量，从而导致更多统一排序覆盖和库复杂程度增加。