[摘要] 目的：利用psbA-trnH间区及内转录间隔区2（inter

[摘要] 目的：利用psbA-trnH间区及内转录间隔区2（internal transcribed spacer 2，ITS2）序列对蕨类植物区系植物进行分子鉴定。方法：对此类植物叶片及药材进行聚合酶链式反应（Polymerase Chain Reaction,PCR)扩增和测序，并从GenBank中下载此类植物样本的psbA-trnH、ITS2序列。所有序列经DNAMAN 8.0软件进行拼接、比对分析，用MEGA 6.0计算相关数据，并建立邻接聚类树（neighbor-joining，NJ）进行样本分析。结果：从NJ聚类树分析可知，所有样本聚为两大支，石松类卷柏属植物聚为一大支，蕨类植物聚为一大支，其中瓶尔小草亚纲植物B.strictum单聚为一支，与水龙骨亚纲植物明显区分，其Bootstrap 支持率较为理想。结论：psbA-trnH间区对蕨类植物的鉴别效率为87.03%，ITS2序列未获得有效序列；psbA-trnH间区及ITS2序列对石松类卷柏属植物的鉴别效率为100%；故而可以证明psbA-trnH间区对蕨类植物区系植物的鉴别具有较好的适用性。
[关键词] 蕨类植物区系；psbA-trnH间区；ITS2；聚类分析

結果 (中文) 3:

[Abstract] Objective: to use psbA-trnH and the internal transcribed spacer (internal 2 transcribed 2 spacer, ITS2) sequence for molecular identification of fern flora. Methods: polymerase chain reaction to this kind of plant leaves and herbs (Polymerase Chain, Reaction, PCR) psbA-trnH, ITS2 amplification and sequencing, sequence and download such plant samples from the GenBank. All sequences were analyzed by DNAMAN 8 software, and the data were calculated by MEGA 6, and the neighbor-joining (NJ) was established. Results: from the phylogenetic tree analysis shows that, for all samples poly into two major branches, clubmosses Selaginella genus species were clustered into one branch, fern poly is a big branch, of which the adderstongues subclass of plant B.strictum monomer as a, with water keel plant subclass distinct, the bootstrap support to rate ideal. Conclusion: trnH intergenic region of ferns identification efficiency was 87.03%, ITS2 sequences did not get effective sequence; trnH intergenic region and the ITS2 sequence of lycopods Selaginella genus identification efficiency was 100%; therefore it can trnH intergenic region of Pteridophyte Flora in plant identification is proved to be applicable.[keyword] pteridoflora; psbA-trnH; ITS2; cluster analysis