Acute toxicity of plastic leachates was analyzed in three exposure experiments.In the first treatment, embryos together in a 50 mL glass Petri dish containing 20 mL of FSW or 20 mL of serial dilution of plastic leachates at 0.05, 0.10, 0.50, 1, and 10 g L−1, respectively. Petri dishes were covered and placed in an incubator at 28 °C in dark.After 1 h, the embryos in each dish were fixed with 2 mL of formaldehyde solution and fertilization was observed under a microscope.The fertilization rate (FR, %) was calculated for fertilized eggs among the examined embryos in 5 mL of treatment sample.In the second treatment, fertilized eggs were exposed in FSW or different concentrations of plastic leachates (0.05, 0.10, 0.50, 1, and 10 g L−1) for 24 h in a glass Petri dish.After 24 h, embryo hatching was detected in each Petri dish.Deformity and shell height of D-veliger larvae were recorded in the samples.