DSC experiment were performed with

DSC experiment were performed with a differential scanning calorimeter . All scans were carried out with a scan rate of 30/h in the temperature range between 25◦Cand75◦C. before measurement the samples were degassed and equilibrated for 15 min before heating. Two heating and cooling cycles were performed .We have examined freshly prepared liposomes,frozen liposomes immediately after thawing and freeze-dried liposomes after re-suspension in purified water .In general ,re- suspension was completed within 5 min by repeated vortexing and heating above the phase transition temperature of the main lipid component(about60◦C). However, to test whether the samples were fully hydrated after this short period, we have performed experiments with longer re-suspension times up to 45 min with 10 min inter-vals.For all DSC experiments a molar lipid to carbohydrate ratio of 1:10(about 1% (w/v) carbohydrate ) was used .Immediately before measurements ,the total lipid content was adjusted to 1 mg/ml with Tris-buffer, which was also employed as reference.
Calorimetric enthalpies (Hcal) were calculated after baseline adjustment and normalization to the phospholipid concentration, by integrating the peak areas using the MicroCal LLC Origin soft-ware ( OriginLab Corporation ,Northampton ,MA,USA).The actual transition temperatures
Were determined at the peak maxima in the heat capacity functions andT1/2Was derived from the half-width of the transition peak indicative for the cooperativity of the transition.

結果 (中文) 1:

用差示扫描量热仪进行了 DSC 实验。所有的扫描扫描率为 30/h 之间 25◦Cand75◦C 的温度范围内进行。在测量之前样品脱气和平衡加热前 15 分钟。进行了两个加热和冷却的循环。我们已检查新鲜的脂质体，解冻后立即冷冻的脂质体冻干的脂质体后再悬浮在纯净水中。一般情况下，再悬浮在 5 分钟内完成了反复的涡流和加热到高于相变温度的主要脂质 component(about60◦C)。然而，为了测试是否样品被充分水化后这段短时间，我们执行时间为 45 分钟与 10 分钟除 vals 停牌时间较长的重新试验。因为所有的 DSC 实验 1:10(about 1%(w/v) 碳水化合物碳水化合物比摩尔脂质) 被使用。紧接前测量，总脂含量被调整为 1 毫克/毫升，也作为参考的三缓冲区。基线调整和磷脂浓度、 归一化后通过集成使用画图 LLC 起源软件 （OriginLab 公司、 美国马萨诸塞州北安普顿市） 的峰面积计算了热焓 (Hcal)。实际转变温度确定了在高峰期的热容量极大值函数 andT1/2Was 来自指示性的协同性过渡转型期峰的半宽度。

結果 (中文) 2:

DSC实验中使用差示扫描量热计进行。所有的扫描用的30 /小时25◦Cand75◦C之间的温度范围内的扫描速率下进行。测量前将样品脱气并平衡为加热前15分钟。两个加热和冷却循环进行。我们有检查新鲜制备的脂质体，解冻后立即进行冷冻干燥重新悬浮在纯化水中。一般来说后脂质体，重新悬浮液通过重复涡旋和以上加热5分钟内完成冷冻的脂质体主要脂质成分（about60◦C）的相变温度。然而，为了测试是否样品充分水化这个短时期后，我们进行了实验较长的停牌重组时间长达45分钟与10分钟间vals.For所有DSC实验的摩尔脂类和碳水化合物的比例1:10 （约1％（重量/体积）的碳水化合物）溶液.Immediately用于测量之前，总脂质含量调节至1毫克/毫升用Tris-缓冲液，将其也作为参考。
量热焓（Hcal）进行了计算后基线调整和标准化的磷脂浓度，通过整合使用MicroCal LLC来源软洁具。实际转变温度的峰面积（OriginLab公司，北安普敦，MA，USA）
分别在该热容量的函数的峰值极大值确定and T1 / 2Was从表示为过渡的协同的转变峰的半宽的。

結果 (中文) 3:

用差示扫描量热仪进行DSC实验。所有的扫描与扫描速度30 /小时，温度在25◦cand75◦C.在测量样品脱气和平衡15分钟，加热前进行。两个加热和冷却循环。我们研究新鲜制备的脂质体的脂质体，冷冻解冻后立即和冻干脂质体在纯化水再悬浮后，重新悬浮在5分钟内完成，通过反复的涡流加热以上的主要脂质成分的相转变温度（大约60◦C）。然而，测试样品是否被充分水化，这在短期内，我们已经进行了实验与再悬浮的时间为45分钟和10分钟inter-vals.for所有DSC实验摩尔脂质1:10的碳水化合物的比例（约1%（W/V）碳水化合物）的使用。之前测量，总脂含量调整为1毫克/毫升的Tris缓冲液，这也是作为参考。热焓（HCAL）基线调整和规范化的磷脂浓度进行计算后，通过积分峰面积利用Microcal公司起源软件（OriginLab公司、北安普敦、马、美国）的实际转变温度。在热容量功能，从指示协同的过渡过渡的半峰宽源t1/2为峰极大值的测定。